Phospho-ampk substrate motif
WebJan 10, 2024 · The 109 phosphosites phosphorylated at higher levels in WT AMPK than in AMPKα1/α2-DKO cells were separated into groups 1 (with the conserved AMPK substrate motif) and 2 (without the motif). WebJan 10, 2024 · We found that the phospho-AMPK substrate motif antibody, which is more specific than the phospho-Ser antibody, was able to detect MFF phosphorylation. However, the phospho-AMPK substrate... We would like to show you a description here but the site won’t allow us.
Phospho-ampk substrate motif
Did you know?
WebAnalysis of phospho-AMPK substrate motif antibody immunoprecipitates by MS/MS. (A, B) Primary hepatocytes were isolated from male C57BL/6 mice (12-week-old). Hepatocytes were incubated with ... WebApr 11, 2024 · In LNCaP cells, inhibition of GSK3β by LiCl led to increased phosphorylation of its substrate glycogen synthase (pGS ... TPD52 characteristic D2-motif, PEST sequences, ... The results showed that the AICAR an activator of AMPK by phosphorylation significantly downregulated TPD52 expression in both LNCaP and VCaP cells. While investigating ...
WebFeb 16, 2024 · A previous quantitative phospho-proteomics study uncovered an overall comprehensive regulatory role of AMPK during DDR with multiple newly identified substrates (Chen et al., 2024), while the intact role and related mechanism of AMPK in DSBR remained unknown.TALEN (transcription activator-like effector nuclease) technology was … WebPTMScan® Phospho-AMPK Substrate Motif (LXRXXS*/T*) Kit Description: PTMScan® Technology employs a propri-etary methodology from Cell Signaling Technology for …
WebAMPK is a highly conserved sensor of intracellular adenosine nucleotide levels that is activated when even modest decreases in ATP production result in relative increases in … WebNov 14, 2024 · Here, we provide evidence that PLIN3 is a novel and direct substrate of AMPK and PLIN3 phosphorylation by AMPK promotes LD dispersion, which implicates PLIN3 in lipolysis pathways. PLIN3 and PLIN2 are less efficient than PLIN1 at inhibiting basal lipolysis (Patel et al., 2014; Sanders et al., 2015 ).
WebJan 10, 2024 · The phosphosites that were upregulated in WT cells but did not meet these rules and did not have sequences similar to the conserved AMPK substrate motif constituted group 2 ( n = 77). These sites may be regulated by the AMPK-dependent signaling pathways but are not directly phosphorylated by AMPK.
WebAMP-activated protein kinase (AMPK) is a conserved energy sensor that plays roles in diverse biological processes via phosphorylating various substrates. Emerging studies … did jack the ripper dieWebWe systematically explored the effects of the AMPK-MFN2 signaling axis on dynamic changes of mitochondria, autophagy and MAMs in response to energy stresses. Results Energy stress promotes MAM formation, autophagy and mitochondrial fission did jacob chansley get released from prisonWeb21 hours ago · AMPK has previously been shown to phosphorylate RabGAPs directly thereby inducing an intramolecular autoinhibition . In mammalian cells, the AMPK-mediated phosphorylation of Ser168 near the N-terminus of TBC1D17 reduces its GAP activity toward its substrate Rab5 . Due to the structural similarities between TBC1D17 and TBC-7, and … did jaclyn smith abandon her childrenWebMay 1, 2015 · AMP-activated protein kinase (AMPK) is a key cellular energy sensor and regulator of metabolic homeostasis. Although it is best known for its effects on … did jacob wrestle with jesusWebNov 3, 2015 · We provide an AMPK motif matrix and a pipeline to predict additional AMPK substrates from quantitative phosphoproteomics datasets. As AMPK is emerging as a … did jaclyn smith have cancerWebApr 25, 2008 · Comparing the optimal motif we identified from the peptide library screen to all known well-established in vivo substrates of AMPK shows excellent concordance (Figure 1 B).Each of these substrates contains not only the required basic residue in −3 or −4, and hydrophobic residues in −5 and +4, but they also exhibit strong bias toward the secondary … did jacob walk with a limpWebTable: PTMScan®Results,Phospho-AMPK Substrate Motif (LXRXXS*/T*), SILAC Study design: Human non-small cell lung cancer (H1650) cell line; LysC/post-IAP trypsin digest; Antibody: Phospho-AMPK Substrate Motif (LXRXXS*/T*), PTMScan®Kit ##5564 Treatments: Untreated (Light), Phenformin-treated (Heavy) 2 did jacob chansley plead guilty