WebSep 1, 2007 · The CFSE high cells were pulsed with OVA I peptide, whereas the CFSE low cells were pulsed with Mut 1 peptide and served as internal controls. These peptide-pulsed target cells were washed extensively to remove free peptides, and then i.v. coinjected at a 1:1 ratio into the immunized mice 6 days after immunization. WebApr 26, 2011 · The percentage of specific lysis was determined using J774 cells (H2 d) that had been pulsed with OVA 257–264 peptide as target cells. Immune spleen cells (effectors) were incubated with target cells at the effector-to-target-cell ratios (E/T Ratio) indicated on the x - axis.
Mature dendritic cells pulsed with exosomes stimulate efficient ...
WebFeb 6, 2024 · Alloreactive T cells target their inducing alloantigens as well as third-party alloantigens but generally fail to target self-antigens. Certain residues located on the alpha-1/2 domains of class I antigen-presenting molecules primarily interface with TCR. WebMar 21, 2024 · The Bi 2 S 3 @OVA@DC can rapidly and durably accumulate in draining lymph nodes and thus trigger stronger T-cell responses compared to OVA-pulsed DCs. Meanwhile, Bi 2 S 3 @OVA@DC can further achieve in vivo antitumor effects against OVA-expressing B16F10 melanoma when combined with fractionated radiotherapy, resulting … lowes carpet kicker rental
Breaking tolerance with engineered class I antigen …
WebMay 24, 2024 · The cytolytic killing of target cells by CTLs requires perforin-mediated release of granzymes, ... IL-1β release from LPS-primed and ova-pulsed BMDCs or EG7 tumour cells co-cultured with OT1-CTLs. WebOct 23, 2024 · Optical coherence tomography angiography (OCTA) is a novel, non-invasive imaging tool used to detect vascular flow. The absence of a flow signal in OCTA in … WebMay 21, 2004 · After stimulation by OVA peptide for 3 days, viable effector T cells were cocultured with target cells EG7 or EL-4 either pulsed with OVA peptide or left unpulsed, at a ratio of 10:1 in a 5-hour ... lowes carpet for sale